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Get information about the safe and proper use of autoclaves.

All autoclave users are responsible for the safe and proper use of these devices. Autoclaves use steam and pressure to sterilize liquids and other media, glassware, instruments and waste. At UB many autoclaves are shared resources that are subject to users of various levels of experience. The videos below are intended to assist users  in understanding how autoclaves function and how to best use an autoclave.

Large Autoclaves

The typical large capacity autoclaves have 3 cycles; liquids, gravity and vacuum.  The video below is an excellent tutorial on the proper use of large capacity autoclaves:

Small Autoclaves

Smaller scale autoclaves usually have one or two cycles, slow exhaust or fast exhaust.  This video describes the proper use of small scale autoclaves and which cycle to use:

Contact an Expert

headshot of david pawlowski.

David Pawlowski, Ph.D, RBP

Biosafety Officer

Environment, Health & Safety

Phone: 716-829-5816