University at Buffalo (UB)
MASS SPECTROSCOPY
Proteomics & Bioanalysis
Located on the Buffalo Niagara Medical Campus, UB's Proteomics and Bioanalysis Core specializes in proteomics, PK/PD, lipidomics, metabolomics and biopharmaceutical analysis. The Core is a resource for UB researchers and regional investigators, providing state-of-the-art instrumentation and expertise in proteomics and bioanalysis. We assist companies of all sizes, from startups to Fortune 500, with programs tailored to fit their needs. Partners trust our services from concept to completion.
Specializing in proteomics, liquid chromatography, mass spectrometry and bioinformatics
Whether your project is large or small, we help with the identification and quantification of proteins and proteoforms present in a biological sample. We enable insights into the complexities and emergent properties of biological systems for drug discovery and development activities. After an initial discussion about your requirements, we will confirm how we can help. If you'd like to proceed, we'll assign an expert team and project manager.
The facility has several main research foci:
• Ultra-sensitive quantification of small-molecule analyses, such as drugs, metabolites and biomolecules
• Comprehensive protein expression profiling and differential proteome analysis
• Ultra-sensitive quantification of proteins/peptides
SERVICES
Protein identification
- Digestion with trypsin or alternative enzymes
- Sample preparation with RPLC and retention time determination in chromatogram
- Peptide fragmentation (HCD) and m/z determination in MS/MS spectrum
- Peptide/protein identification with database searching software
Protein characterization
- Post-translational modifications (PTMs): phosphorylation, deamidation, methylation, nitration, acylation
- Other: cleavage site determination, amino acid substitution, disulfide crosslinking, chemical modifications
Quantitative proteomics
- Label-free quantitative LC-MS/MS
- Quantification of whole proteome in samples
- > 100 sampling capacity
- Efficient and reproducible on-pellet digestion
- Separation on long nano LC column
- High-resolution MS on Orbitrap Fusion Lumos
- Accurate quantification models
Data analysis packages
- Protein/peptide a PTM identification: Proteome Discoverer (SEQUEST/MASCOT), MSGF+, PEAKS, Maxquant
- Peptide filtering a protein grouping: IDPicker, Scaffold
- Proteome-wide quantitative analysis: SIEVE, Skyline, DIANN, MaxQuant
Technologies
Micro-scaffold Assisted Spatial Proteomics (MASP) - precisely procures spatial information by precise tissue spatial compartmentalization using a 3D-printed micro-scaffold, and accurately/sensitively quantifies thousands of proteins in a whole-tissue slice.
Ion-current-based MS1 global proteomic quantification Ion (IonStar) - an MS1-based quantitative method devised for large-cohort proteomics analysis, which prominently alleviates issues associated with quantitative precision, missing data and false-positive discovery of protein changes.
State-of-the art instrumentation
The Core consists of two instrument rooms, totaling 600 sq ft, housing state-of-the-art liquid chromatography (LC) systems, mass spectrometer (MS) and sample processing instruments:
- Thermo Orbitrap Astral mass spectrometer
- Thermo TSQ Altis triple quadrupole mass spectrometer
- Thermo TSQ Quantiva triple quadrupole mass spectrometer
- Thermo Dionex Ultimate 3000 nano/micro HPLC systems with WPS-3000 autosamplers
- Waters Bioseparation semi-preparation HPLC system
- Several additional nano/micro LC systems (i.e. Eksigent nano-2D, Agient 1200-series micro HPLC, Shimadzu AC20 HPLC)
Put us to work for you
Our team is dedicated to providing comprehensive and cost-effective services to UB's scientific community, partner institutions and industry collaborators. We welcome collaborations on both research projects and commercial endeavors. Contact us to request a consultation.
COMPANIES WE WORK WITH
Additional life sciences resources available to our partners
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