NEXT-GENERATION SEQUENCING FOR DISCOVERY & GROWTH
Genomics & Bioinformatics
Located on the Buffalo Niagara Medical Campus and the first next-generation sequencing service provider in the region, UB's Genomics and Bioinformatics Core Facility provides high-quality services including massively parallel sequencing, single cell technologies, bulk transcriptome, epigenome, microbiome and spatial transriptome platforms. Our team is dedicated to providing comprehensive and cost-effective genomic/transcriptomic and bioinformatics services to UB's scientific community, partner institutions, and industry collaborators.
State-of-the art instrumentation and services in genomics & bioinformatics to fuel your research
The UB Genomics and Bioinformatics facility leverages the latest genomic platforms and services, as well as dedicated bioinformatics experts, to serve your R&D goals. We will work with you to customize solutions to help you get one step closer to your commercialization goal.
FROM SAMPLE TO RESULTS
State-of-the art instrumentation and expertise in genomics Et bioinformatics to fuel your research.
We perform nucleic acid extraction, library prep, sequencing and bioinformatics.
Next-Generation Sequencing Services
The UB Genomics and Bioinformatics core provides all next generation sequencing services from nucleic acid extraction through bioinformatics analysis. We provide expert consultation services to help design your sequencing project to optimize data produced for your project. From bacteria to human and all organisms in between, we offer a range of methodologies for our providers including:
- Automated, high-throughput nucleic acid purification
- DNA/RNA/miRNA Quality Control Services
- Next Generation Sequencing Library preparation (WGS, WES, RNAseq)
- High Throughput Sequencing (short read and long read)
- Epigenomics using bulk ATAC-Seq, ChIP-Seq, Cut&Run, Cut&Tag, Methylation Capture
- Single Cell Transcriptomics, Single Cell Immune Profiling, Single Cell Multiome (scGEX and scATACseq)
- Microbiome 16S V1-V3 and V3-V4 sequencing and Shotgun Metagenomics
- Genome Assembly (Hi-C, HiChIP)
- Spatial Transcriptomics using VISIUM and Xenium platforms
- Project Consultation/Budget proposals/Bioinformatics Analysis
Spatial Biology
10X Genomics XENIUM Platform – RNA in situ platform using fresh/frozen or fixed formalin paraffin embedded (FFPE) tissue sections to identify transcript localization at the sub cellular level within human or mouse tissues. Current methods use smaller gene probes (~300 genes) targeted to specific tissue types (brain, lung, etc) or large gene probe sets (~5000) across multiple tissues to identify RNA targets within your tissue of interest.
10X Genomics VISIUM – Unbiased, species agnostic RNA expression platform to identify transcripts expressed at near single cell resolution within tissue sections of your choice. We can use both fresh/frozen tissue (OCT embedded) or FFPE material in our standard or high definition protocols.
Sequencing
The UBGBC uses short read sequencers from Illumina and long read Oxford Nanopore systems to meet your needs.
Illumina NovaSeq – Facilitates projects of any size, from large-scale single-sell sequencing efforts to whole genome and de novo genome assembly projects. Enabled by our direct connection to UB’s Center for Computational Research, we can process samples rapidly and provide timely results for your projects.
Illumina NextSeq – Rapidly processes bulk RNA-Seq, ChIP-Seq, Exome-Seq, and other similar experiments with a capacity up to 1.7 billion reads.
Illumina MiSeq – Specializes in more targeted sequencing geared toward smaller genomes. This includes targeted genomic amplicons and bacterial 16S sequencing.
Oxford Nanopore MinION – Long read sequencing platform for smaller project needs from bacterial16S microbiome to human whole genome analysis.
Single-Cell Sequencing
The 10x Genomics Chromium X platform specializes in single-cell RNA-Seq, single-cell V(D)J, single nuclei ATACseq, and single nuclei multiomics that are compatible with our Illumina sequencers. In addition, we offer other single cell platforms (Illumina Single Cell 3’) for high throughput projects with reduced costs in mind. With a capacity to capture 200 to 1 million cells or nuclei, we can fulfill all your single cell needs.
3’ and 5’ Transcriptome – The 10X Genomics Chromium X system can process 3’ or 5’ gene expression profiles from hundreds to thousands of cells or nuclei. The Illumina Single Cell 3’ system can capture up to 1 million cells and provide cost effective 3’ transcript expression for your individual cell types.
Immune Profiling - The 10X Genomics platform has the ability to perform V(D)J immune profiling experiments for mouse and human models. The Genomics core can provide cell isolation using Miltenyi Biotech MACS antibodies as needed for your project.
Epigenomics- The 10X Genomics portfolio can assay chromatin accessibility at single-cell resolution. The combination of ATAC-Seq with the 10X single-cell barcoding system allows for researchers to gain insight into the roll chromatin conformation has a roll in DNA-protein binding.
Multiomics – The 10X Genomics system can capture both 3’ gene expression and chromatin accessibility within the same single nuclei, providing unparalleled access to unlock transcriptional regulatory pathways.
Bioinformatics Services
The core facility is well equipped to handle a wide array of sequencing analysis, with three dedicated bioinformatics analysts on staff. The GBC leverages UB’s Center for Computational Research (CCR) high-performance compute system to rapidly analyze datasets generated at our facility.
Every research project is unique, therefore we highly encourage reaching out to the core facility for project planning and bioinformatics consultation prior to sequencing. Fee-for-service and collaborations-based models are available depending on the project needs.
Put us to work for you
We welcome collaborations on both research projects and commercial endeavors. Contact us prior to submitting samples, as timing and viability of cells is essential for successful processing. During the consultation period we can address your questions and discuss sample preparation for your sequencing project.
Additional life sciences resources available to our partners
Fuel your research with state-of-the art instrumentation and expertise in proteomics and biopharmaceutical analysis in our Proteomics & Bioanalysis Core.
Our integrated, enterprise-level biorepository program offers biospecimen processing and storage services to support healthcare research and development.
Advance your discoveries and give them room to grow with fully equipped laboratories and specialized amenities in our life sciences incubator spaces.
Access infrastructure and experts in software development, artificial intelligence and data analytics at our Center for Computational Research.