Research News

UB researchers identify new drug target for remyelination in MS

Image shows myelin production by transplanted human oligodendrocyte progenitors cells.

Myelin (green) production by transplanted human oligodendrocyte progenitors cells (red) is greatly improved following the genetic block of M3R expression. Image: Jesslie J. Polanco

By ELLEN GOLDBAUM

Published July 18, 2018

headshot of Fraser Sim
“This work establishes that M3R has a functional role and if blocked, could improve myelin repair. It better positions the field for clinical trials that will be aimed at blocking these receptors in MS patients.”
Fraser Sim, associate professor
Department of Pharmacology and Toxicology

Remyelination, the spontaneous regeneration of the fatty insulator in the brain that keeps neurons communicating, has long been seen as crucial to the next big advance in treating multiple sclerosis (MS). However, a lack of understanding of how remyelination is stymied in the disease has hampered these efforts.

Now, UB researchers have found that a receptor called muscarinic type 3 (M3R), a key regulator of the remyelination process, is a promising drug target. M3R is a muscarinic receptor found on oligodendrocyte progenitor cells (OPCs), the cells that make myelin.

The finding is likely to generate some interest in the pharmaceutical world, since other muscarinic receptors have also been found to be involved in animal models of demyelinating diseases.

“Our data provide evidence that a drug targeting M3R specifically would be a useful strategy,” says Fraser J. Sim, associate professor of pharmacology and toxicology in the Jacobs School of Medicine and Biomedical Sciences at UB and senior author on the paper describing the work.

Published in late June in the Journal of Neuroscience, the research builds on work the team published in 2015 demonstrating that MS occurs when OPCs are unable to mature and differentiate properly. They found that activation of M3R blocked differentiation of OPCs into myelin-making cells.

In that paper, the researchers reported that a drug already on the market for overactive bladder successfully inhibited that receptor, allowing for remyelination to occur in an animal model.

“That work identified solifenacin as a possible drug useful for remyelination, but we really weren’t sure which specific receptor the drug worked on,” Sim explains. Since the drug wasn’t specific to a signal receptor, he says it could produce unwanted side effects in patients.

To single out which receptor was responsible for blocking remyelination, the UB team took a genetic approach to blocking expression of M3R in both human and mouse myelin-making cells. The result was that they prevented OPCs from making M3R at all.

“This work establishes that M3R has a functional role and if blocked, could improve myelin repair,” Sim says. “It better positions the field for clinical trials that will be aimed at blocking these receptors in MS patients.”

Sim has National Institutes of Health (NIH) funding to further understand the mechanisms of action of M3R and how it goes about impairing differentiation of myelin-making cells. “The hope is that this will identify new and more attractive drug targets beyond M3R,” he says. “The grant also is geared toward understanding how the receptors are activated in disease. If we can understand that, then we might have another opportunity for targeting this pathway in MS.”

Funding for the research came from the National Institute of Neurological Disorders and Stroke of the NIH, the National Multiple Sclerosis Society, the Kalec Multiple Sclerosis Foundation, the Change MS Wellness Foundation, the Skarlow Memorial Trust and the Empire State Stem Cell Fund through the New York State Department of Health.

The first authors on the paper are R. Ross Welliver, a medical student, and Jessie J. Polanco, a doctoral neuroscience candidate, both in the Jacobs School. Other UB co-authors are Richard A. Seidman, Anjali K. Sinha, Melanie A. Obara, Zainab M. Khaku, Diara A. Santiago Gonzalez, M. Laura Feltri and Pablo M. Paez. Akiko Nishiyama of the University of Connecticut and Jurgen Wess of the National Institute of Diabetes and Digestive and Kidney Diseases were also co-authors.