Watch a cell defend itself from acid!
Many biological molecules are sensitive to changes in pH and thus changes of pH often cause pathology. Many of the membrane transport proteins that remove acids from cells are pH dependent, being allosterically activated by intracellular protons to help normalize cell pH. Our lab is interested in determining the pH dependence of a transporter that moves alkali (bicarbonate) into cells to neutralize intracellular acid. We hypothesize that it should also be activated by intracellular protons.
We are looking for a student to make wild-type and mutant versions of this transporter, generate RNA, and inject RNA into a model cell (frog egg) and monitor its activity while we experimentally vary pH. This is a chance to learn something new about how cells defend themselves from acid.
The specific outcomes of this project will be identified by the faculty mentor at the beginning of your collaboration.
Data will be fit to a equation to determine parameters of pH dependence. Results will be presented at lab meeting and as a poster at a local meeting.
|Length of commitment||About a year (10-12 months)|
|In-person, remote, or hybrid?||In person|
|Level of collaboration||Individual student project|
|Who is eligible||All undergraduate students who have taken a PGY course|
Once you begin the digital badge series, you will have access to all the necessary activities and instructions. Your mentor has indicated they would like you to also complete the specific preparation activities below. Please reference this when you get to Step 2 of the Preparation Phase.
Please click the link below to read the review the journal article from The Molecular Aspects of Medicine: