Chromatography and Spectrometry

Liquid Chromatography Mass Spectrometer (LCMS) – Agilent Technologies 6470

Zoom image: Agilent 6470 QQQ Liquid Chromatography Mass Spectrometer Agilent 6470 QQQ Liquid Chromatography Mass Spectrometer

Agilent 6470 QQQ Liquid Chromatography Mass Spectrometer

The Agilent 6470 Triple Quadrupole LC/MS system is at the forefront of robustness, reliability and accuracy, which makes it a perfect option for many applications, including peptide quantitation, food safety, environmental, clinical research and forensics. The Agilent 6470 Triple Quadrupole LC/MS delivers superior sensitivity for trace level analysis with performance specifications in signal-to-noise (S/N) and instrument detection limit (IDL). It achieves sensitivity and resolution specifications with autotune.

Features

  • System components:
    • 1260 Infinity II quaternary pump: 800 bar, 5 ml/min flow
    • 1260 Infinity II multisampler: 2 x 54-vial containers
    • 1260 Infinity II multicolumn thermostat
    • 2 pos/6 port Quick Change valve head: 800 bar
    • PEAK Nitrogen Generator System
    • Quiet Cover MS40 mechanical pump
    • 6470 QQQ mass spectrometer
    • MassHunter Workstation software
  • Ion source: electrospray (ESI)
  • Collision cell: curved and tapered hexapole
  • Ion detector: ±20 kV high-energy conversion dynode (HED) w/ high-gain electron multiplier
  • Vacuum system: two turbomolecular pumps with one mechanical pump
  • MRM sensitivity signal-to-noise ratio (S/N):
    • ESI positive: > 75,000:1
    • ESI negative: > 30,000:1
  • MRM sensitivity instrument detection limit (IDL):
    • ESI positive: < 4.0 fg
    • ESI negative: < 4.0 fg
  • Mass range: m/z 5 – 3,000
  • Polarity switching: 25 ms
  • Mass resolution: autotune 0.7 Da, manual tune 0.5 Da
  • Mass accuracy:
    • 0.14 Da for m/z 5 – 999
    • 0.20 Da for m/z 1,000 – 1,999
    • 0.30 Da for m/z 2,000 – 3,000
  • Mass stability: ≤ 0.1 Da in 24 hours
  • Dynamic range: > 6.0 × 106
  • Scan modes: MRM, SIM, MS scan, product ion scan, neutral loss/gain scan, and precursor ion scan
  • MRM transitions: 450 per time segment, up to 13,500 MRM transitions per method
  • Dynamic MRM transitions: Up to 4,000 dynamic MRM transitions per method
  • Triggered MRM transitions: Up to 10 MRM transitions (primary and secondary) per analyte for library search and compound confirmation
  • Maximum scan rate: 17,000 Da/s
  • Maximum MRM acquisition rate: 500 MRMs/s
  • Minimum MRM dwell time: 0.5 ms
SOP_Agilent 6470 QQQ LC-MS_Regular Operation_01_24_2020.pdf

Location

Materials Characterization Laboratory
Furnas Hall room #109/110
University at Buffalo North Campus
Buffalo, NY 14260

Hours of operation

24/7

Fees

Category C Rates

  • Internal Academic: $15 per hour
  • External Academic: $27 per hour
  • Industry: $60 per hour
Learn more about our rates
For general inquiries, contact:

Donald J. Goralski
Director, Shared Instrumentation Laboratories
(716) 645-5151

For technical inquiries, contact:

Zongmin (Shirley) Bei, PhD
Senior Research Support Specialist
Shared Instrumentation Laboratories (716) 645-5165