Published March 8, 2013
A paper by
senior author Gabriela
K. Popescu, PhD, associate professor of biochemistry, has
been selected and evaluated by the Faculty of 1000, placing her work in
its library of the top 2 percent of articles in biology and
The article received two out of three stars—or a
“very good” rating—by an evaluator with the the
post-publication peer review service.
The paper sheds light on processes involving the synaptic
protein NMDA receptor, whose overactivity has been implicated in
neurodegenerative conditions and mental illness.
Upon binding the neurotransmitter glutamate, this protein gates an ionic current across the cellular membrane, Popescu explains. The current is necessary for normal development of the central nervous system as well as learning and memory throughout life.
However, under certain conditions, such as stroke or ischemia,
the NMDA receptor produces an enhanced current that leads to
The research addresses unknown questions about how currents are produced in response to glutamate binding and how this response can be modulated.
Popescu and her colleagues studied basic receptor properties in the protein’s C-terminal domain—one of two with recognition sites for neuromodulators.
When they produced and engineered receptors that lacked portions
of their C-terminal tails, they found that truncated receptors had
profoundly altered functions.
Using more selective truncations of the C-terminal domain, they
determined that the N1 tail altered how much current each opening
produced, whereas the N2 tail determined how much the receptor
“This result clearly indicated that each N1 and N2 tail has distinct roles in controlling the NMDA receptor current,” notes Popescu.
“This is important because, in vivo, each of the N1 and N2 tails is modified by phosphorylation, and thus responds to intracellular cascades.”
Popescu’s paper “provides new insight into
intracellular mechanisms of (NMDA) receptor modulation,”
according to F1000 reviewer Robert Peoples, PhD, associate
professor of biomedical sciences at Marquette University.
His full F1000 evaluation is available online.
Bruce E. Maki, a PhD candidate in neuroscience, is lead author on the paper, which was originally published in the Journal of Biological Chemistry.
Co-authors are Teresa K. Aman, a postdoctoral associate in
biochemistry, and former PhD students Stacy A. Amico-Ruvio
and Cassandra L. Kussius.
To be recommended for F1000
Prime, a research article has to have been recognized as being
“of special excellence,” says senior managing editor
“Articles are selected for recommendation purely on the
basis of their scientific merit and regardless of the journal in
which they are published.”
F1000 prime highlights and recommends articles across biology
and medicine based on the opinions of more than 5,000 expert
scientists and clinical researchers.
The service subdivides biology and medicine into principal subject areas, including pharmacology and drug discovery.
“Each faculty has its own panel of peer-nominated experts
who recommend the best of the literature relevant to their subject
area,” Delamare says.