Alyssa Le: Investigating Differences in Aversive Behavior in LOF Nuclear Calcium Sensors as Compared to Wild-Types

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This project involved investigating the differences in aversive behavior in LOF nuclear calcium sensors as compared to wild-types. Through this experience, Alyssa developed independent research skills that will be utilized towards gaining a PhD in science.  Here is what Alyssa had to say about the experience:

What I Did:

"I aided in Dr. Denise Ferkey's laboratory, becoming proficient in designing primers for PCR, outcrossing C. elegans, and confirming homozygous alleles for a deletion through gel electrophoresis. From these preliminary steps, I proceeded on to behavioral assays in different mutant strains compared to wild-type (N2) animals in hopes of varied aversive behaviors to specific genetic differences. My contribution was for a much larger project which aims to identify genes responsible for aversive behavior in C. elegans through studying variation in this trait using wild isolate animals."

"I am most proud of my learned ability to design my own primers which allowed me to optimize the experiments to what I needed. My biggest takeaway from this project was that science is not linear and through the many failed PCR reactions and gels, I was able to show grit and perseverance. From being in this lab, I have learned that I want to pursue my PhD, and there is no doubt that the skills and connections I have made will aid in my professional and academic goals. This experience had made me a more receptive scientist that is able to communicate projects effectively and concisely."

What I Learned:

"The experiences that I have gained from this project and being involved in the lab overall is almost immeasurable.  I had previous experiences with c. elegans and research, however, this was the first taste of independent research without the guiding hand of company-created lab kits that had no other outcome but to work perfectly. I learned how to maintain the c. elegans and about their lifespans much more in depth than any previous class I had taken. I had learned how to outcross mutant strains and to assay. I gained critical thinking skills and confidence in myself as an independent scientist. I will utilize not only the hard science skills I have learned, but also the communication and troubleshooting skills in future schooling, scientific research and my career."