Dipti Kalshetti: Therapeutic Biophotonics for Regenerative Medicine

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This project involved using Lasers and LEDs to modulate pain or inflammation and promot tissue healing. Dipti focused specifically on exploring wound closure dynamics in cells. Here is what Dipti had to say about the experience:

What I Did:

"My project focused on investigating wound closure dynamics in a keratinocyte cell (HaCaT) monolayer, with a specific emphasis on wound healing. The approach involved leading a team of six individuals in conducting experiments using an in vitro model of cell migration. We utilized laser treatments with various wavelengths (457 nm, 490 nm, 570 nm, 660 nm, 810 nm, 940 nm, and 1064 nm) and employed the 'Einstein' modular method to identify the most effective wavelength for accelerating cell healing processes. Preliminary findings indicated the superior efficacy of a specific wavelength, prompting further validation through loss-of-function and gain-of-function experiments."

What I Learned:

"The impacts of our research extend beyond the lab. By pinpointing a wavelength that enhances cell healing, our findings hold promise for advancing wound care and treatment modalities. I'm most proud of working with a diverse team while leading and navigating the challenges of complex experiments, fostering collaborative success. From this experience, my leadership and problem-solving skills flourished. I learned to delegate effectively, enhanced my proficiency in laboratory techniques, and embraced resilience when faced with unexpected hurdles. The biggest takeaway is the fusion of technical expertise and teamwork—essential in scientific pursuits."