Have a part of your teeth chipped apart after chewing on something too hard? It was probably an expensive treatment to get it fixed. Hi, my name is Kevin Le and I am a senior at the University at Buffalo. I have been mentored by Dr. Rosemary Dziak since Summer of 2019. Our lab focuses on the use of Nano-calcium sulfate in combination with other factors in search of improving bone regenerative therapy. A previous student had shown a positive trend in osteoblastic cells viability in combination with nCS and 1,4-DPCA using MTT assay. My responsibilities this past year was to determine the relationship between alkaline phosphatase level and the combination of nCS + a drug called 1,4-DPCA using an Alkaline Phosphatase Assay. Researches have been experimenting with calcium sulfate for quite some time now but there is not much known about Nano-calcium sulfate due to its size difference. By experimenting, it shows the importance of how the size difference of nano-calcium sulfate can be used differently in smaller areas such as teeth. To better our understanding, we hope to determine the efficiency of nCS in combination of 1,4-DPCA in an Alkaline Phosphatase Assay.
Nano-calcium sulfate is a bone graft material that improves bone regeneration by enhancing differentiation of osteoblastic cells. 1,4-dihydrophenonthrolin carboxylic acid (1,4-DPCA) is a drug that was tested in vivo mouse wound healing. It has been shown to stabilize a protein that promotes growth factor expression and enhanced regeneration. In combination of the two factors, an increase of osteoblastic cells activity was shown on an MTT assay reading as concentration of 1,4-DPCA increases. The incubation period of 48 hours has shown to be significant where a large percentage of 1,4-DPCA was released. As for the Alkaline Phosphatase assay, activity showed a biphasic effect on osteoblastic cells with low 1,4-DPCA concentration.
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