Properties of a New H+ Channel in the Cornea of the Eye

image of single-channel events in an excised patch.

This image shows the single channel events recorded from an excised segment of Xenopus frog oocyte membrane using the patch-clamp technique which will be used in this project.

Be the first to characterize a newly discovered ion channel in the cornea of the eye.

Project description

The cornea of the eye is a transparent structure that allows the passage of light into the eye. The cornea contains a collagen matrix stroma whose transparency is partially determined by corneal endothelial cells which pump fluid out of the stroma. We are studying the role of SLC4A11, an endothelial cell membrane transporter, in this process. We hypothesize that the SLC4A11 protein is an H+ channel. A student investigator in this project will express SLC4A11 protein in Xenopus frog eggs and then use the excised-patch clamp technique to examine membrane patches from these oocytes for evidence of H+ channel events.

Project outcome

At the end of this project the student will design a poster that will present the project background, goals, data and conclusions. In addition, data acquired by the student may be used in a scientific journal article.

Project details

Timing, eligibility and other details
Length of commitment Longer than a semester (6-9 months)
Start time Anytime
Level of collaboration Individual student project
Benefits Academic credit
Who is eligible Juniors and Seniors
Support Funding is available after the completion of the preparation badge. See below.

Core partners

  • D. Mark Parker, Associate Professor of Physiology and Biophysics

Project mentor

Michael Duffey

Professor

Department of Physiology and Biophysics. Jacobs School of Medicine & Biomedical Sciences.

Office: 4154. Lab: 4150. 955 Main St. Buffalo, NY 14203

Phone: (716) 829-3111

Email: duffey@buffalo.edu

Preparation

To engage with this project, you will work through a series of Project-Based Collaboration digital badges to guide your experience. These badges will:

  1. Guide you in setting your goals and intentions as well as gaining additional context,
  2. Enhance your collaboration with your mentor/partner,
  3. Support your reflection and help you integrate your learning with your academic and professional goals.

All students who wish to participate in a project on the Project Portal must first complete the Project Preparation Digital Badge. First, contact the project mentor to discuss availability and after you’ve received approval, you can start the Project Preparation Digital Badge.

Preparation guidance

Once you have registered for the Project Preparation Digital Badge, you will receive further instructions related to the activities you will need to complete. In addition, you will also complete the following preparation activities for this particular project: