Properties of a New H+ Channel in the Cornea of the Eye

image of single-channel events in an excised patch.

This image shows the single channel events recorded from an excised segment of Xenopus frog oocyte membrane using the patch-clamp technique which will be used in this project.

Be the first to characterize a newly discovered ion channel in the cornea of the eye.

Project description

The cornea of the eye is a transparent structure that allows the passage of light into the eye. The cornea contains a collagen matrix stroma whose transparency is partially determined by corneal endothelial cells which pump fluid out of the stroma. We are studying the role of SLC4A11, an endothelial cell membrane transporter, in this process. We hypothesize that the SLC4A11 protein is an H+ channel. A student investigator in this project will express SLC4A11 protein in Xenopus frog eggs and then use the excised-patch clamp technique to examine membrane patches from these oocytes for evidence of H+ channel events.

Project outcome

At the end of this project the student will design a poster that will present the project background, goals, data and conclusions. In addition, data acquired by the student may be used in a scientific journal article.

Project details

Timing, eligibility and other details
Length of commitment Longer than a semester (6-9 months)
Start time Anytime
Level of collaboration Individual student project
Benefits Academic credit
Who is eligible Juniors and Seniors
Goldwater and the National Science Foundation

Students participating in this project might be interested in and eligible for the Goldwater Scholarship and the National Science Foundation Graduate Research Fellowship. Connect with the Office of Fellowships and Scholarships to learn more.

Core partners

  • D. Mark Parker, Associate Professor of Physiology and Biophysics

Project mentor

Michael Duffey

Professor

Department of Physiology and Biophysics. Jacobs School of Medicine & Biomedical Sciences.

Office: 4154. Lab: 4150. 955 Main St. Buffalo, NY 14203

Phone: (716) 829-3111

Email: duffey@buffalo.edu

Start the project

  1. Email the project mentor using the contact information above to express your interest and get approval to work on the project. (Here are helpful tips on how to contact a project mentor.)
  2. After you receive approval from the mentor to start this project, click the button to start the digital badge. (Learn more about ELN's digital badge options.) 
Fulfilling Academic Major/Minor Requirements

If you are planning to use this project to satisfy program requirements for your academic major or minor, it is your responsibility to obtain approval from your academic department prior to beginning the project. 

Preparation activities

Once you begin the digital badge series, you will have access to all the necessary activities and instructions. Your mentor has indicated they would like you to also complete the specific preparation activities below. Please reference this when you get to Step 2 of the Preparation Phase.