Structure-Function studies on NSAID-targeting enzymes.
This project is not being offered for the current term. Please check back next semester for updates.
The cyclooxygenase enzymes (COX-1 and COX-2) catalyze the committed step in the biosynthesis of prostaglandins. COX-1 and COX-2 are the pharmacological targets of nonsteroidal anti-inflammatory drugs (NSAIDs), such as aspirin and ibuprofen, while COX- 2 is selectively inhibited by Celebrex and Vioxx. The objective is to evaluate how sequence differences between COX-1 and COX-2 influences the differential regulation of COX catalysis and inhibition observed between isoforms. The student will utilize kinetic and biophysical characterizations of mutant forms of COX-1 and COX-2 to evaluate sequence differences in specific regions of each enzyme.
The goal is to expose undergraduate students to biomedical research by providing them with hands-on experiences. A student will be extensively trained and specifically tasked with techniques involved generating mutant enzyme constructs, overproduction and isolation of mutant enzymes and subsequent functional characterization of the enzymes. Research results will be formally presented within the Departmental seminar series at the end of the project period. The expectation is for the student to present research findings in poster format at a research symposium held on campus or at a neighboring Institution.
Length of commitment | Longer than a semester; 6-9 months |
Start time | Spring (January/February 2025) |
In-person, remote, or hybrid? | In-Person Project (Can only function with in-person engagement) |
Level of collaboration | Individual Student Project |
Benefits | Stipend |
Who is eligible | Juniors & Seniors; background in introductory courses in chemistry and biochemistry is preferred |
Michael Malkowski
Professor and Department Chair
Structural Biology
Phone: (716) 829-3698
Email: mgm22@buffalo.edu
Once you begin the digital badge series, you will have access to all the necessary activities and instructions. Your mentor has indicated they would like you to also complete the specific preparation activities below. Please reference this when you get to Step 2 of the Preparation Phase.
structural biology, biochemistry, Jacobs School of Medicine and Biomedical Sciences