Introduction to Laboratory Skills

2013 fellows hard at work.

Introduction to Laboratory Skills (1-Week Mini Course) Instructors: Dr. David Shubert/Debbie Timineri

CLIMB UP Fellows participate in a 1-week intensive laboratory training course to prepare them with skills they will utilize in their summer research projects and in the future. Students will take a Laboratory Safety training, Lab Animal Facility and Rodent Handling Orientation, and a half-day intensive on Responsible Conduct of Research. 

 

Date/Time/Room

 

 

Topics Discussed

   May 31st (Wednesday)

   1:30-3:00pm

   235 BEB

 

 

Laboratory Safety Training (Tony Oswald, EHS)

 

   June 1st (Thursday)

   8:30am-12pm: Group 1

   1:00pm-4:30pm: Group 2

   235 BEB

General Waste Practices

Autoclaving

Calculations

Labelling Samples

Laboratory Notebooks

   June 1st (Thursday)
   8:30am- 10:30am: Group 2 
   2:00pm-4:00pm: Group 1

   134B Farber

 


Responsible Conduct of Research and Ethics Training (Dr. David Hoekstra)

   June 2nd (Friday)

   8:30am-12pm: Group 1

   1:00pm-4:30pm: Group 2

   235 BEB

Buffer Preparation

Weighing

Measuring Liquids

pH meter

 

   June 5th (Monday)

   8:45am-12pm: Group 1 (LAF)

   8:30am-12pm: Group 2 (Proteins)

   12:45pm-4:30pm: Group 1 & 2 (LAF)

   235 BEB (then 119 LAF)

Rodent Handling & Euthanasia

LAF Orientation

 

 

 

 

  June 6th (Tuesday)

   8:30am-12pm: Group 1

   12:45pm-4:30pm: Group 2 (LAF)

   235 BEB

 

 

Pipetting

Protein Assay

Spectrophotometer

Microsoft Excel

   June 7th (Wednesday)

   8:30am-12pm: Group 1

   1:00pm-4:30pm: Group 2

   235 BEB

 

Sterile Technique

Cell Counting

Trypan Blue Exclusion Assay

 

   June 8th (Thursday)

   8:30am-12 pm: Group 1

   1:00pm-4:30pm: Group 2

   235 BEB

 

Sodium Dodecyl Sulfate Poly Acryilamide Gel Electrophoresis (SDS-PAGE)

   June 9th (Friday)
   8:30am-12 pm: Group 1

   1:00pm-4:30pm: Group 2

   235 BEB

De-stain Gel

H and E staining

Review