Date Established: 6/16/2010
Date Last Updated: -
Category: Environmental, Health and Safety
Environment, Health and Safety
Associate Vice President for Facilities
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The purpose of this policy is to provide guidance for disposing of wastes generated as a result of gram staining procedures performed in a laboratory setting. These wastes are considered hazardous by the USEPA and must be properly collected.
This policy applies to all university laboratories, research areas, and any other spaces serviced by UB Environment, Health and Safety.
Refer to Hazardous Chemical Waste Management Guidebook.
The primary responsibility for following this policy rests with the principal investigator or supervisor of the area where this procedure is being performed. Any laboratory researcher, student, or other employee who do gram staining and mange hazardous wastes in these areas must be trained and made aware of this policy. If there is any doubt or question as to the applicability of this policy to their specific area, contact EH&S for further assistance.
Gram staining is used to differentiate bacterial species into two large groups based on their chemical and physical properties. Generally three chemical reagents are used during the process; a crystal violet indicating solution, ethanol, and fuchsin indicating solution. Water may also used as a rinsing agent. The gram staining process requires a microscope slide inoculated with a sample possibly contains bacteria to be treated and rinsed a number of times using the reagents listed above.
A waste that poses substantial or potential threats to public health or the environment. These wastes must be collected and disposed per strict Environmental Protection Agency (EPA) regulations. Ethanol in particular is a flammable material and must be collected as a hazardous waste.
This procedure shall be reviewed once every two years, or as changes require.
|Revision||Section(s) Changed||Change(s) Made
|1||All||Reformatted for web||1/2018|
|Environment, Health and Safety (EH&S)||716-829-3301|