Feltri Research Overview

Our research focuses on adhesion between myelinating cells, axons and the extracellular matrix and the signals that promote myelination. One of our major efforts has been the study of laminin receptors on Schwann cells, the myelinating peripheral glia. By generating and comparing animal models of demyelinating neuropathies to patient's biopsies, we and others have determined that laminins are required for 'radial sorting' of axons in early development. Radial axonal sorting is a pre-requisite for myelination and is arrested in human laminins and dystroglycan-glycosyltransferases deficiencies. Using conditional mutagenesis we have determined that integrins, dystroglycan and RhoGTPAses are required for radial sorting because they induce cytoskeletal rearrangements that allow the generation of glial extensions that contact and wrap axons.

More recently, we have adapted innovative sub-fractionation and proteomic techniques to profile the extensions contacting axons and the RhoGTPAse interactome in Schwann cells. By these techniques we have identified novel molecules important for myelination and for the support of axons by glial cells. We have recently discovered that mechanical forces generated by the extracellular matrix and other cells are also essential for correct myelination, and we are actively pursuing the molecular mechanisms by which mechanical signals are transduced in myelin-forming glia.  

Laminin receptors are also important for myelin and nodes of Ranvier to achieve the correct length, thickness, architecture and stability. Patients lacking laminins have abnormally thick and instable myelin, short internodes and immature nodes of Ranvier. We first determined that laminin 211, dystroglycan and certain integrins are required to form myelin of normal cytoarchitecture, and now we are seeking to understand why perturbing laminin function leads to myelin instability and demyelination.

Using genetic, cell biology and biochemistry we are testing the hypothesis that laminin receptors influence growth factors and signaling molecules to prevent demyelination. These studies also lead us to discover that some of the signaling molecules under scrutiny are more important in the central nervous system, where they inhibit oligodendrocyte myelination. Thus, they represent potential molecular targets to promote remyelination in demyelinating disease such as Multiple Sclerosis of Leukodystrophies.

Since our arrival at the HJKRI we are applying our experience on conditional mutagenesis to ask if there is cell autonomy in the pathogenesis of Krabbe disease.

Faculty and Staff

Dr. M. Laura Feltri

Professor of Biochemistry & Neurology

Dr. Feltri is currently Professor of Biochemistry and Neurology at the Hunter James Kelly Research Institute in the State University of New York at Buffalo.  Before 2011 she was the Head of the Unit of NeuroGlia in the San Raffaele Scientific Institute of Milano, and adjunct Associate Professor in the Department of Neurology at the University of Pennsylvania.

Yannick Poitelon

Research Assistant Professor, Department of Biochemistry

Post Doctoral Research Scientist, HJKRI, Feltri Lab

  • 2016 - present: Hunter James Kelly Research Institute, University at Buffalo, NY, USA, Research Assistant Professor
  • 2011 - present: Hunter James Kelly Research Institute, University at Buffalo, NY, USA, Postdoctoral Research Scientist
  • 2010 - 2011: DIBIT - Neuroglia Unit, San Raffaele Institute, Milan, Italy, Postdoctoral fellow
  • 2008: Vascular Biology Unit, Instituto de Biomedicina de Valencia, Spain, Training
  • 2005 - 2009: PhD in Medical Genetic, Aix-Marseilles University, France, "Exploration of animal and cellular models of AR-CMT2A"
  • 2004 - 2005: Master in Human Pathology, Aix-Marseille University, France. "Pathophysiological mechanisms of Lamin A/C associated Charcot-Marie-Tooth disease"

Leandro Marziali

Post Doctoral Research Scientist, HJKRI, Feltri Lab

  • 2016 - present: Postdoctoral Research Scientist in the Feltri laboratory at the Hunter James Kelly Research Institute for studying the role of p38gamma on myelination and oligodendrocyte maturation.
  • 2016: received PhD in neuroscience.
  • 2010 - 2016: Laboratory of Dra. Juana Pasquini at the School of Pharmacy and Biochemistry of the University of Buenos Aires (IQUIFIB-CONICET). PhD project involving the study of the combined effects of Transferrin and Thyroid Hormone on CNS myelination and oligodendrocyte maturation.
  • 2009: University of Buenos Aires, Buenos Aires, Argentina. Biochemistry major.

Kathleen Catignas

  • 2011 - present: Hunter James Kelly Research Institute, SUNY Buffalo, Department of Biochemistry. PhD student under Dr. M. Laura Feltri, investigating the integrins at the Schwann cell-axon interface during peripheral nerve development.
  • 2007 - 2011: University of North Carolina @ Greensboro. Lab instructor. Member of Dennis LaJeunesse lab, studying the role of DH31-expressing enteroendocrine cells in the Drosophila midgut.
  • 2002 - 2007: Florida State University. Biology major with a concentration in Cellular/Molecular Biology. Florida Bright Futures Scholar. 2007 Undergraduate Teaching Assistant. Directed Individual Study in Drosophila genetics with Dr. Wu-Min Deng.

Nadav Weinstock

MD-PhD student, HJKRI, Feltri Lab

  • 2012 - present: MD/PhD student, University at Buffalo School of Medicine
  • 2008 - 2012: BS, Biological Sciences, University at Buffalo

Gustavo Della Flora Nunes

PhD student, HJKRI, Feltri Lab

  • 2015 - present: Hunter James Kelly Research Institute, SUNY Buffalo, Department of Biochemistry. PhD student under Dr. M. Laura Feltri
  • 2014 - 2015: Master in Neuroscience, Federal University of Rio Grande do Sul, Brazil. “Animal model of autism by prenatal exposure to valproic acid: analysis of excitatory and inhibitory synapses”
  • 2009 - 2013: BS, Biomedical Sciences, Federal University of Rio Grande do Sul, Brazil

Yoonchan Hwang

Research Technician III, HJKRI, Feltri lab

  • 2014- present: Research Technician III in the Feltri laboratory at the Hunter James Kelly Research Institute for studying the role of p38gamma on myelination and oligodendrocyte maturation.
  • 2014: BS, Biological Science, University at Buffalo
  • 2013-2014: Laboratory of Dr. Michael C. Yu, Biological Science, University at Buffalo. The projects involve arginine methylation in protein-protein interactions in the TRAMP complex and splicesomal proteins in S. cerevisiae.

Students

Irene Yu, Medical Student

Arsalan Haghdel, Undergraduate student, Biomedical Sciences

Thomas Rush, Undergraduate Student, Biochemistry

Past Members

  • Kevin Espino, Undergraduate
  • Scott Ferguson, Undergraduate
  • Gustavo Della Flora Nunes, Undergraduate
  • Marta Pellegatta, PhD Student
  • Dominique Ameroso, Master student & Research Technician
  • Kansho Abiko, Undergraduate
  • Monica Ghidinelli, PhD Student
  • Marilena Palmisano, PhD Student
  • Kenneth Minorczyk, Undergraduate