Analyze the tissue from treated animals in a cancer study to determine if there was any off-target antibody accumulation.
Our patented humanized antibody, hJAA-F11, appears promising with specificity for human carcinomas including those of breast, lung, prostate, colon, bladder and ovary, as shown by our recently published paper concerning specificity of JAA-F11 by immunohistochemistry and in vivo imaging (Karacosta et al. 2018). The efficacy of the two different hJAA-F11 constructs, H2aL2a and H3L3, was shown in mouse models of human triple negative breast cancer, human non-small cell lung cancer and human small cell lung cancer. No apparent pathology of normal tissues was seen.
To continue safety validation, the tissues of these treated mice must be analyzed for unexpected off-target antibody accumulation. This analysis will be performed by immunohistochemistry, a method in which tissue samples on microscope slides are analyzed for human antibody deposition.
A poster presentation is the expected outcome of the student's work on this project. This work might be also be included in a publication.
|Length of commitment||A semester|
|Start time||Winter, Spring, Summer|
|Level of collaboration||Individual student project|
|Benefits||Academic Credit, Volunteer, Work Study|
|Who is eligible||Any undergraduate student|
Once you begin the digital badge series, you will have access to all the necessary activities and instructions. Your mentor has indicated they would like you to also complete the specific preparation activities below. Please reference this when you get to Step 2 of the Preparation Phase.