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Jun                            Qu

Jun Qu PhD

Department of Pharmaceutical Sciences

Associate Professor

 
Professional Summary:

Research focus areas: Proteomics and Pharmaceutical Analysis. Major research programs in the proteomic field involve i) high-resolution and large-scale expression profiling of pathological proteomes (e.g. for cardiovascular diseases, colon cancer and infectious diseases) for the discovery of disease/therapeutic biomarkers by gel-free LC/MS methods; ii) Sensitive identification, localization and quantification of post-translational modifications in complex proteomes, with the emphases on arginine methylation and phosphorylation. Novel anti-PTM-peptides capture procedure and alternating collision induced dissociation (CID)/electron transferring dissociation (ETD) are employed to obtain abundant PTM information; iii) targeted quantification of regulatory, marker proteins for clinical study. Dr. Qu‘s lab possesses many state-of-the-art LC/MS instruments, including a high resolution/accuracy LTQ/Orbitrap XL with ETD, a highly sensitive TSQ Quantum Ultra EMR triple-quadrupole instrument, two ultra-high pressure nano-LC systems, and several HPLC instruments for pre-fraction and ion chromatography. A number of key analytical advances have been developed by his lab that greatly enhanced the proteomic coverage, sensitivity and throughput for proteomic research. As for the Pharmaceutical Analysis of small-molecule drug/markers, Dr. Qu‘s lab is focusing on the ultra-sensitive quantifications of drug, metabolites and endogenous markers (e.g. corticosteroids, di-hydroxyl-vitamin D metabolites, androgens, etc.) using a novel combination of selective enrichment and micro- or nano- LC/MS.

Education and Training:
  • PhD, Tsinghua University (2002)
  • BS, Tsinghua University (1997)
Employment:
  • Adjunct Associate Professor, Roswell Park Cancer Institute (2013-present)
  • Associate Professor, Pharmaceutical Sciences, University at Buffalo, department of Pharmaceutical sciences (2012-present)
  • Adjunct Assistant Professor, Biochemistry, University at Buffalo, Department of Biochemistry (2011-present)
  • Adjunct Assistant Professor, Ophthalmology, University at Buffalo, Department of Ophthalmology (2010-present)
  • chief scientist, New York Center of Excellence in Bioinformatics and Life ScienceNew York Center of Excellence in Bioinformatics and Life Science (2007-present)
  • Graduate Faculty, Pharmaceutical Sciences, University at Buffalo, department of Pharmaceutical sciences (2007-present)
  • Assistant Professor, Pharmaceutical Sciences, University at Buffalo, department of Pharmaceutical sciences (2009–2013)
  • Research Assistant Professor, Pharmaceutical Sciences, University at Buffalo, department of Pharmaceutical sciences (2004–2008)
Awards and Honors:
  • Outstanding contribution award for medicinal analysis (2015)
  • Thermo Scientific Research Award (2015)
  • Selected by the Chemistry Department of Tsinghua University as one of the ten most prominent alumni graduated 2000-2010. (2012)
  • Delegate of the International Task Force for Vitamin D and MS Prevention (2011)
  • Fellow of Buffalo Clinical and Translational Research Center (2011)
  • American Heart Association Scientist Development Award (2011)
  • Clinical and Translational Award of SUNY (2010)
  • Chinese Association for Instrumental Analysis (2004)
  • outstanding academic delegate and met with Chinese President Jiang Zhemin (2001)
  • Chinese-Glories Scholarship (2000)
  • Dr. Mei Yiqi Scholarship (1999)
  • Dupont Distinguished Student Scholarship (1998)

Research Expertise:
  • • Pharmaceutical Analysis:: Targeted quantification of regulatory, marker proteins for clinical study, using highly-sensitive nano-LC/SRM-based methods. A number of key analytical advances have been developed in his laboratory, which greatly enhance the proteomic coverage, sensitivity and throughput for proteomic research.
  • • Proteomics:: i) high-resolution and large-scale expression profiling of pathological proteomes (e.g. for cardiovascular diseases, colon cancer and infectious diseases) for the discovery of disease/therapeutic biomarkers using gel-free LC/MS methods; ii) Sensitive identification, localization and quantification of post-translational modifications in complex proteomes; Here, novel anti-PTM-peptide capture procedures, and alternating collision induced dissociation /electron transferring dissociation methods are employed to obtain abundant PTM information;
Grants and Sponsored Research:
  • September 2015–June 2020
    Transcription mechanism of Myrf for central nervous system myelination.
    NIH
    Role: Co-Investigator
  • October 2015–September 2019
    Dynamic Remodeling From Reversible Ischemia and Sudden Cardiac Arrest
    Department of Veterans Affairs
    Role: Co-Principal Investigator
  • September 2015–August 2019
    ER stress and diabetic retinopathy
    NIH/NEI
    Role: Co-Principal Investigator
  • August 2014–May 2019
    Function of Regulator of G protein signaling in aging skeleton
    NIH/NIA
    Role: Co-Investigator
  • June 2014–May 2019
    Regulation of skeletal development and homeostasis by IFT protein
    NIH/NIDCR
    Role: Co-Investigator
  • January 2015–December 2018
    PTPN14 and YAP tyrosine modification regulate the YAP oncogenic function
    American Cancer Society (ACS)
    Role: Co-Principal Investigator
  • December 2015–November 2017
    A high-throughput LC/MS method for quantification of biotherapeutics
    Roche-Pharmaceuticals
    Role: Principal Investigator
  • September 2012–July 2017
    Gamma-hydroxybutyrate: Toxicokinetics, Toxicodynamics and Treatment Strategies
    NIDA
    Role: Co-Investigator
  • June 2014–May 2017
    Vitamin D and Children’s Diabetes
    Murdoch Childrens Research Institute
    Role: Principal Investigator
  • December 2014–December 2016
    Study of the ER-mitochondria interface as a new target in diabetic retinopathy
    NIH, NEI
    Role: Co-Investigator
    $438,188
  • January 2012–December 2016
    Biomarker Release after Reversible Ischemia
    AHA
    Role: Principal Investigator
  • September 2015–August 2016
    Characterization of ADC in tissues and study of mAb catabolism/metabolism
    Peer-reviewed Industry Consortium Funds
    Role: Principal Investigator
  • July 2013–June 2016
    Enrichment Toolkit for Proteomic Biomarkers
    UB
    Role: Principal Investigator
  • June 2014–May 2016
    Investigation of the utility of LC/MS for characterization of the plasma and tissue PK of a novel series of antiCEA monocolonal antibodies.
    Hoffmann-La Roche Inc
    Role: Co-Investigator
  • January 2011–December 2015
    Biomarker Release after Reversible Ischemia
    American Heart Association
    Role: Principal Investigator
    $385,000
  • December 2014–October 2015
    Extensive investigation of protein binding in lipsome preparations
    Merrimack Pharma
    Role: Principal Investigator
  • June 2014–May 2015
    Brain network: Membrane permeable transcriptional regulators for retinal repair
    Center for Hearing and Deafness
    Role: Principal Investigator
  • May 2010–April 2015
    Protein arginine methylation in trypanosomes
    NIH NIAID
    Role: Co-Principal Investigator
    $250,000
  • May 2006–April 2015
    Metabolic Adaptation and Functional Recovery of Hibernating Myocardium
    NIH
    Role: Co-Investigator
  • September 2012–August 2014
    Subcellular domains of myelinating-glia: capturing axonal contact
    NIH
    Role: Co-Principal Investigator
    $435,875
See all (10 more)

Journal Articles:
See all (58 more)

Professional Memberships:
  • American Heart Association (2011)
  • AAPS (2011)
  • American Association of Pharmaceutical Scientists (AAPS) (2011–present)
  • Human Proteome Organization (HUPO) (2011–present)
  • American Heart Association (AHA) (2011–present)
  • International Society of Heart Research (ISHR) (2010–present)
  • American Society of Mass Spectrometry (2004)
  • American Society for Mass Spectrometry (ASMS) (2004–present)
Presentations:
  • "Global proteins with high reliability, accuracy and precision" EAS annual meeting (2016)
  • "High-resolution MS1 approach for selective and sensitive quantification of large cohorts: less label, more free" invited talk, ThermoFisher Scientifics (2016)
  • "IonStar: a promising alternative to SWATH for large-scale protein quantification" Invited seminar (sponsored by ThermoFisher Scientifics), ThermoFisher Scientifics (2016)
  • "LC/MS characterization of biotherapeutics" Invited Seminar, Amgen Inc, Amgen Inc (2016)
  • "Extensive and Reproducible Proteomic Measurements in Large Biological Cohorts with High Accuracy, Precision and Low Levels of Missing data" 2016 Mass Spectrometry Symposium, University of Rochester (2016)
  • "A promising alternative to SWATH:MS1-based strategy(IonStar) enables in-depth and reproducible proteomics quantification in large cohorts with high accuracy and extremely-low-level-missing-data" 64th American Society for Mass Spectrometry Annual Meeting (2016)
  • "A Trapping micro-LC/MS strategy for ultra-sensitive, robust and high-throughput quantification of biotherapeutics and biomarkers in complex matrices" 64th American Society for Mass Spectrometry Annual Meeting (2016)
  • "Global and targeted protein quantification" 64th American Society for Mass Spectrometry Annual Meeting (2016)
  • "Use of reagent-free LC/MS for sensitive analysis of biotherapeutics" Boehringer-Ingelheim Protein Analysis Symposium (2016)
  • "LC/MS for global and targeted protein analysis" invited seminar talk, Dept. Chemistry, SUNY-Buffalo (2016)
  • "In-depth and Reproducible Proteomic Measurement in Large Pharmaceutical and Clinical Cohorts with High Accuracy and Low Missing Value" invited talk, Dept. Pharmaceutical Sciences, U. Wisconsin (2016)
  • "label free quantification with low missing data and high accuracy" 2016 Annual Conference for Association of Biomolecular Resource Facilities, Association of Biomolecular Resource Facilities (2016)
  • "LC/MS quantification of low-abundance proteins: is this real? Are we there yet?" Invited Webinar talk, Chinese Association of Analytical Techniques (2015)
  • "LC/MS, a promising alternative for biotherpeutics analysis" invited talk, Bristol-Myers Squibb (2015)
  • "A Promising Alternative to SWATH for Biomarker Discovery: An Ion-Current Based Approach for Large-Scale, Accurate and Extensive Proteomic Quantification with Extremely Low Level of Missing Data" 2015 Eastern Analytical Symposium (2015)
  • "Challenges and strategies for quantitative characterization of biotherapeutics" 5th Pharmaceutical Analysis Conference (2015)
  • "Clinical and pharmaceutical proteomics: are we there yet?" invited seminar, Department of Chemistry, Tsinghua University (2015)
  • "Investigation of protein drugs by LC/MS: how to deal with the complexity and sensitivity issues" invited speaker, Department of Medicine, Tongji Medical School (2015)
  • "LC/MS analysis of proteins and peptides by LC/MS" 2015 Eastern Analytical Symposium (2015)
  • "Ultra-Sensitive and Accurate Analysis of Biotherapeutics Using Low-Flow LC/MS" 2015 Eastern Analytical Symposium, Eastern Analytical Symposium (2015)
  • "A novel method for reproducible measurement of proteins in large-scale investigation" invited seminar, Dept. of Biology, University of Vermont (2015)
  • "A promising alternative to SWATH: an Ion-current-based strategy with extensive proteomic coverage and extremely low level of missing data" BCEIA conference 2015 (2015)
  • "Competing risk factor of heart failure vs. sudden cardiac arrest by proteomics" HUPO Human Proteome Organization World Congress, Human Proteome Organization World Congress (2015)
  • "The gaps between biomarker candidates and clinical biomarkers" 2015 Euromedlab Annual meeting, The International Federation of Clinical Chemistry and Laboratory Medicine (2015)
  • "low flow LC/MS for biotherapeutics analysis" Abbvie Inc. meeting, Abbvie Inc. (2015)
  • "Analysis of protein drugs with LC/MS" Abbvie Inc. meeting, Abbvie Inc. (2015)
  • "Proteomics for unbiased neurological investigation" SUNY Brain Network of Excellence Workshop, SUNY (2014)
  • "Bioanalysis of proteins and peptides by LC/MS" 2014 Eastern Analytical Syposium, 2014 Eastern Analytical Syposium (2014)
  • "LC/MS analysis of therapeutic proteins and peptides" 2014 Conference of American Association of Pharmaceutical Scientists, ASMS (2014)
  • "Large-Scale Pre-Clinical/Clinical Profiling”" HUPO Human Proteome Organization World Congress, Human Proteome Organization (2014)
  • "Retinal proteome profiling for ROP using high-end LC/MS" NIH RPDP meeting (2014)
  • "Highly Sensitive Quantification of Bio-therapeutics Using Nano- and Micro-LC/MS" AB SCIEX Worldwide Webinar Series, AB SCIEX (2014)
  • "Clinical and Pharmaceutical Proteomics: Global and Targeted" Buffalo Pharmaceutics Symposium (2014)
  • "Drastically improved sensitivity and robustness for quantification of therapeutic mAb in tissue and application in PK studies at therapeutically-relevant dosing levels" Center of Protein Therapeutics Symposium (2014)
  • "Multiplexed quantification of antigen-antibody complex, free antibody and antigen in plasma and application in PK of CEA and an anti-CEA mAb (T84.66) in a xenograft tumor model" Center of Protein Therapeutics Symposium (2014)
  • "High-throughput proteomics analysis for retinopathy" NIH Webinar, NIH Webinar (2014)
  • "Characterization of biotherapetuics using cutting-edge LC/MS techniques" Janssen Pharmaceutical, Janssen Pharmaceutical (2014)
  • "Towards the sensitive and accurate analysis of biotherapeutic with Low-flow LC/MS" Roche Pharmaceutical, global, Roche Pharmaceutical (2013)
  • "Towards a Highly Sensitive and Accurate Characterization of Therapeutic mAb in Plasma and Tissues using Low-flow LC/MS" 2013 Conference of American Association of Pharmaceutical Scientists (2013)
  • "Towards the highly sensitive and accurate quantification of mAbs" Fifth Annual Symposium, Center for Protein Therapeutics (2013)
  • "Towards the highly sensitive and accurate quantification of mAbs" Center of Protein Therapeutics Symposium (2013)
  • "Use of LC-MS to measure Biotherapeutics in biological samples" 2nd BioTx ADME Workshop, Cosponsored with AAPS (2013)
  • "Does first year MRI and clinical activity correlate with long term clinical response to glatiramer acetate in multiple sclerosis patients" American Academy of Neurology, American Academy of Neurology (2013)
  • "Proteome-level Pharmacokinetic/Pharmacodynamic Analysis of Corticosteroid-responsive Proteins in Rat Liver" 2012 AAPS Annual Meeting and Exposition, AAPS (2012)
  • "An innovative dual-enzyme and dual-activation strategy for comprehensive and accurate characterization of protein arginine-methylation in Trypanosoma brucei" HUPO 2012 11th Annual World Congress, HUPO (2012)
  • "Clinical investigation of potential biomarkers for innate HIV-1 virus control in long-term-non-progressing patients using a comprehensive and reproducible ion-current-based proteomic expression profiling" HUPO 2012 11th Annual World Congress, HUPO (2012)
  • "Comparative proteomic analysis of isolated colon cancer cells from multiple patients using a comprehensive ion current based approach" HUPO 2012 11th Annual World Congress, HUPO (2012)
  • "Differential proteomic profiling of bronchoalveolar lavage fluid (BALF) for biomarker discovery in COPD" HUPO 2012 11th Annual World Congress, HUPO (2012)
  • "Extensive, proteome-level investigation of the corticosteroid pharmacodynamics with 12 time points using a reproducible and comprehensive ion-current-based profiling strategy" HUPO 2012 11th Annual World Congress, HUPO (2012)
  • "Investigation of the action mechanisms of new anti-carcinoma agents via high-resolution, comparative analysis of the drug-responsive proteomic signatures" HUPO 2012 11th Annual World Congress, HUPO (2012)
  • "Ultra-sensitive biomarker investigation and comprehensive proteomics profiling of drug-responsive proteins using low-flow LC/MS" XenoBiotic Laboratories, XenoBiotic Laboratories (2012)
  • "Biomarker discovery in chronic obstructive pulmonary disease: comparative proteome analysis of bronchoalveolar-lavage-fluid using a comprehensive and reproducible ion-current-based method" 60th ASMS conference on Mass Spectrometry and Allied Topics, ASMS (2012)
  • "Characterization of the Kinetics of Therapeutic Antibody Immune Complexes In Vivo Using Blue Native Electrophoresis and Targeted Nano-LC/MS Analysis" 60th ASMS conference on Mass Spectrometry and Allied Topics, ASMS (2012)
  • "Extensive, Proteome-Level Investigation of the Corticosteroid Pharmacodynamics with 12 Time Points Using a Reproducible and Comprehensive Ion-Current-Based Profiling Strategy" 60th ASMS conference on Mass Spectrometry and Allied Topics, ASMS (2012)
  • "Identification of Novel HIV-invasion-markers by Comparing Long-term-non-progressor vs. Normal-progressors among HIV-infected patients using comprehensive and reproducible ion-current-based proteomic expression profiling" 60th ASMS conference on Mass Spectrometry and Allied Topics, ASMS (2012)
  • "Ultrasensitive, high-throughput and Robust Quantification of monoclonal antibodies in tissues and plasma using a novel Trapping-micro-LC/MS strategy and OAO optimization" 60th ASMS conference on Mass Spectrometry and Allied Topics, ASMS (2012)
  • "accelerated Method Development for Sensitive, Accurate and Reproducible Quantification of Therapeutic Monoclonal Antibodies in Various Tissues Using Orthogonal-array-optimization and Nano-LC/SRM-MS" 2011 American Society for Mass Spectrometry annual meeting (2011)
  • "An innovative dual-enzyme and dual-activation strategy for comprehensive and accurate characterization of protein arginine-methylation in Trypanosoma brucei." 2011 American Society for Mass Spectrometry annual meeting (2011)
  • "An ion-current-based, comprehensive and reproducible proteomic strategy for comparative characterization anti-cancer agents in a prostate cell model" 2011 American Society for Mass Spectrometry annual meeting, ASMS (2011)
  • "An ion-overfilling approach that significantly improves the sensitivity of Orbitrap and application in highly sensitive and accurate proteomic profiling" 2011 American Society for Mass Spectrometry annual meeting (2011)
  • "An ion-overfilling approach that significantly improves the sensitivity of Orbitrap and application in highly sensitive and accurate proteomic profiling" 2011 American Society for Mass Spectrometry annual meeting (2011)
  • "Investigation of the effect of cocaine use and withdrawal in rat models using comprehensive and reproducible ion-current-based proteomic expression profiling" 2011 American Society for Mass Spectrometry annual meeting (2011)
  • "Large-scale comparative proteomic analysis of isolated colon cancer cells from multiple patients using a comprehensive and reproducible ion-current-based method" 2011 American Society for Mass Spectrometry annual meeting (2011)
  • "Molecular-level elucidation of the anti-carcinoma mechanisms of new agents via high-resolution, comparative analysis of the drug-responsive proteomic signatures" 2011 American Society for Mass Spectrometry annual meeting (2011)
  • "Retina Proteome Profiling in a Rat Model of Smith-Lemli-Opitz Syndrome Using a comprehensive, precise and reproducible Ion-current-Based Method" 2011 American Society for Mass Spectrometry annual meeting (2011)
  • "Cutting edge LC/MS-based method for investigation of Ophthalmological proteome" (2011)
  • "Accurate quantification and validation of therapeutic macromolecules" "The Delaware Valley DRUG METABOLISM DISCUSSION GROUP Symposium" (2010)
  • "Targeted investigation of mAB by nano-LC/MS/MS" (2010)
  • "Ultra-sensitive quantification of therapeutic proteins" Buffalo Pharmaceutical Syposium (2010)
  • "A comprehensive analysis of Swine Plasma Proteome Using Combinatorial Peptide Ligand Libraries (CPLL), Dual-Enzyme Digestion and Dual-Activation sequencing with LTQ/Orbitrap/ETD" 2011 American Society for Mass Spectrometry annual meeting (2010)
  • "A high resolution, highly reproducible and sensitive nano_LC configuration that is particularly suitable for large-scale label-free expression profiling" 2010 American Society for Mass Spectrometry annual meeting (2010)
  • "A rapid, reproducible, on-the-fly orthogonal array optimization method for targeted protein quantification by LC/MS and its application in clinical analysis" 2010 ASMS Anuual Meeting (2010)
  • "A rapid, reproducible, on-the-fly orthogonal array optimization method for targeted protein quantification by LC/MS and its application in clinical analysis" 2010 ASMS Anuual Meeting (2010)
  • "Exploring Cyclic Peptide Fragmentation by Electron Transfer Dissociation Coupled to Orbitrap Mass Spectrometry" 2010 American Society for Mass Spectrometry annual meeting (2010)
  • "Vitamin D and cognitive impairment in multiple sclerosis (MS)" American Academy of Neurology (2010)
  • "Vitamin D metabolites are linked to clinical and MRI outcomes in multiple sclerosis patients" American Academy of Neurology (2010)
  • "Photoaffinity labeling studies to better define the mechanism of action for Phase II oncology drug KX2-391 (KX01)" 239th National ACS Meeting, San Francisco, CA, March 22, 2010 (2010)
  • "Identification Of Cysteine Residues In ALDH2 Responsible" Experimental Biology 2009, FASEB (2009)
See all (68 more)
Service Activities:
  • Pharmacy Practice, School of Pharmacy Curriculum Committee; Member (2011)
  • Runing the proteomics and pharmaceutical analysis facility; Assist and provide expertise and resource to the faculty member in PHC and other departments for proteomic analysis and pharmaceutical analysis. collaborated with >20 groups.; Other (2009)
  • Running the COE proteomics core facility; Other

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Contact Information

318 Kapoor Hall
Buffalo, NY 14214-8033
Phone: (716) 645-4821
Fax: (716) 645-4821
Email: junqu@buffalo.edu


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