Date Established: 8/27/2009
Date Last Updated: -
Category: Environmental, Health and Safety
Environment, Health and Safety
Associate Vice President for Facilities
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The purpose of this policy is to provide guidance for the proper handling and disposal of ethidium bromide containing stock solutions and gels which have not undergone a destaining process. This also includes any contaminated debris.
Policy applies to all university laboratories and research areas serviced by UB EHS where ethidium bromide is in use.
Refer to Hazardous Waste Guidebook, section 5.3.5.
The primary responsibility for following this policy rests with the principal investigator. However, all researchers, graduate students, etc, associated with a particular laboratory area where ethidium bromide is used must be trained and made aware of this policy.
Agent commonly used as a nucleic acid stain in molecular biology. Although ethidium bromide is not considered a RCRA hazardous waste by the USEPA, due to its highly toxic nature UB EHS will collect and dispose of it as such.
Any solutions or gels which have not undergone a destaining process are considered toxic. These materials must be handled and collected as hazardous waste.
Used in destaining process. May be present in the form of “tea bags” or specialized media columns. These must be handled and collected as hazardous waste.
Any wipes, gloves, pipette tips, paper towels, test tubes, etc contaminated with ethidium bromide must be handled as hazardous waste.
This procedure shall be reviewed once every two years, or as changes require.
|Revision||Section(s) Changed||Change(s) Made
|1||All||Reformatted for web||1/2018|
|Environment, Health and Safety (EH&S)